4-Aminoantipyrine

Catalog No.S4509 Batch:S450901

Technical Data

Formula	C₁₁H₁₃N₃O
Molecular Weight	203.24	CAS No.	83-07-8
Solubility (25°C)*	In vitro	DMSO	40 mg/mL (196.81 mM)
		Water	40 mg/mL (196.81 mM)
		Ethanol	40 mg/mL (196.81 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	4-Aminoantipyrine(Ampyrone) is a metabolite of aminopyrine with analgesic, anti-inflammatory, and antipyretic properties. It is used as a reagent for biochemical reactions producing peroxides or phenols.
In vitro	The level of glutathione(GSH) in single erythrocytes is impacted slightly at low AAP(4-aminoantipyrine) concentrations probably due to the protection of glutathione reductase. When AAP concentration further increases, AAP has a significant influence on GSH^[2].
In vivo	4-aminoantipyrine (4-AA) is not genotoxic and mutagenic in vivo, it can interfere with DNA damaging agents biological activities and may reduce the effectiveness of DNA damage-based chemotherapy^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human erythrocytes Concentrations 1 × 10−6 mol/L to 1 × 10−3 mol/L Incubation Time 2 h Method Erythrocytes in PBS are incubated for 2 h at ambient temperature (without mixing) with AAP in concentrations from 1 × 10⁻⁶ mol/L to 1 × 10⁻³ mol/L. Sample of erythrocytes without AAP is used as control. After the exposure of AAP, erythrocytes are washed with borate buffer (pH 9.0) 2 times by centrifuging and determined for GSH.
Animal Study:^{^[1]}	Animal Models Male Wistar rats Dosages 240 µmol/kg Administration iv(caudal vein)

Cell Assay:^{^[2]}

Cell lines
Human erythrocytes
Concentrations
1 × 10−6 mol/L to 1 × 10−3 mol/L
Incubation Time
2 h
Method
Erythrocytes in PBS are incubated for 2 h at ambient temperature (without mixing) with AAP in concentrations from 1 × 10⁻⁶ mol/L to 1 × 10⁻³ mol/L. Sample of erythrocytes without AAP is used as control. After the exposure of AAP, erythrocytes are washed with borate buffer (pH 9.0) 2 times by centrifuging and determined for GSH.

Animal Study:^{^[1]}

Animal Models
Male Wistar rats
Dosages
240 µmol/kg
Administration
iv(caudal vein)

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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