3,3'-Diindolylmethane

Catalog No.S4728 Batch:S472801

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Technical Data

Formula

C17H14N2
Molecular Weight 246.31 CAS No. 1968-05-4
Solubility (25°C)* In vitro DMSO 49 mg/mL (198.93 mM)
Ethanol 18 mg/mL (73.07 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description 3,3'-Diindolylmethane(DIM) is a major digestive product of indole-3-carbinol, a potential anticancer component of cruciferous vegetables. 3,3'-Diindolylmethane is a strong, pure androgen receptor (AR) antagonist.
Targets
Androgen Receptor [1]
In vitro DIM is a potent radioprotector and mitigator that functions by stimulating an ATM-driven DDR-like response and NF-κB survival signaling. DIM can inhibit invasion, angiogenesis, and proliferation and induce apoptosis in tumor cells by modulating signaling pathways involving AKT, NF-κB, and FOXO3. It can also inhibit estrogen-inducible gene expression and cause an endoplasmic reticulum stress response. DIM alters estrogen metabolism by shifting metabolism from carcinogenic 16α-hydroxy to inert 2-hydroxy derivatives, and it antagonizes estrogen and androgen receptor activity[1].
In vivo Preliminary studies show DIM is most effective against total body irradiation (TBI) when given in multiple once-daily doses. Treatment of DIM has radioprotection and mitigation properties in vivo. DIM Activates ATM in Normal Tissues. DIM can be given to mice (250 mg/kg) by oral gavage, with no acute toxicity and excellent bioavailability[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    184A1 and Hs578Bst cells

  • Concentrations

    0.3 μM

  • Incubation Time

    24 h

  • Method

    The 184A1 and Hs578Bst cells are pretreated with DIM (0.3 μM) or vehicle for 24 h, irradiated by using different doses of 137Cs γ rays, harvested, plated at different densities, incubated for 14 d, and counted for colony formation.
Animal Study:[1]
  • Animal Models

    Sprague-Dawley (SD) rats

  • Dosages

    75 mg/kg

  • Administration

    i.p

Selleck's 3,3'-Diindolylmethane has been cited by 2 publications

Tetrandrine attenuates intestinal epithelial barrier defects caused by colitis through promoting the expression of Occludin via the AhR-miR-429 pathway [ FASEB J, 2021, 35(5):e21502] PubMed: 33811696
Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-c-src-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis. [ Cell Death Dis, 2019, 10(2):38] PubMed: 30674869

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.