2-NBDG

Catalog No.S8914 Batch:S891401

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Technical Data

Formula

C12H14N4O8
Molecular Weight 342.26 CAS No. 186689-07-6
Solubility (25°C)* In vitro DMSO 68 mg/mL (198.67 mM)
Ethanol 3 mg/mL (8.76 mM)
Water 2 mg/mL (5.84 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.85mg/ml Taking the 1 mL working solution as an example, add 50 μL of 17 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.4mg/ml Taking the 1 mL working solution as an example, add 50 μL of 68 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 2-NBDG, a fluorescent deoxyglucose derivative, is a a marker for detecting glucose uptake and an indicaotr of cell viability.
In vitro

1.1 Preparation of the stock solution
Dissolve 1 mg of 2-NBDG in 2.92 mL of DDH2O to obtain 1 mM of 2-NBDG.
Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of 2-NBDG working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 10-200 μM of 2-NBDG working solution.
Note: Please adjust the concentration of 2-NBDG working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1,000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of 2-NBDG working solution, and then incubate at room temperature for 5-60 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. .If test viability, recorded the optical density (O.D.) at 540/570 nm. Cell viability was calculated as a control ratio and plotted against the logarithmic concentration of the drug to calculate IC50.
In vivo

2-NBDG fluorescence intensity following 30-minutes topical application was 6-fold and 4-fold higher in OSCC and OED, respectively, compared to normal mucosa. Receiver operator characteristic analysis show 83% sensitivity and 73% specificity for detection of neoplasia vs benign (normal and inflammation). Faster 2-NBDG fluorescence temporal decay in neoplasia indicated higher uptake and glucose metabolic rate than normal mucosa. Mucosal delivery of 2-NBDG by topical application to the in-vivo oral surface is feasible and delineates neoplasia from normal mucosa, providing in-vivo noninvasive molecular imaging of dysregulated glucose metabolism.[2]

Biological Activity

Description 2-NBDG, a fluorescent deoxyglucose derivative, is a a marker for detecting glucose uptake and an indicaotr of cell viability.

Protocol (from reference)

Selleck's 2-NBDG has been cited by 4 publications

The essential roles of m6A RNA modification to stimulate ENO1-dependent glycolysis and tumorigenesis in lung adenocarcinoma [ J Exp Clin Cancer Res, 2022, 41(1):36] PubMed: 35078505
A sensitive and simple HPLC-FLD-based method for the measurement of intracellular glucose uptake [ Food Chem, 2021, 372:131218] PubMed: 34624783
A Novel Mechanism of Cannabidiol in Suppressing Hepatocellular Carcinoma by Inducing GSDME Dependent Pyroptosis [ Front Cell Dev Biol, 2021, 9:697832] PubMed: 34350183
Integrating metabolomic data with machine learning approach for discovery of Q-markers from Jinqi Jiangtang preparation against type 2 diabetes [ Chin Med, 2021, 16(1):30] PubMed: 33741031

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.