(2-Hydroxypropyl)-β-cyclodextrin (HP-β-CD)

Catalog No.S4760 Batch:S476003

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Technical Data

Formula

C63H112O42

Molecular Weight 1541.54 CAS No. 128446-35-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (64.87 mM)
Water 100 mg/mL (64.87 mM)
Ethanol 100 mg/mL (64.87 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description (2-Hydroxypropyl)-β-cyclodextrin (HP-β-CD, HP-β-cyclodextrin, Hydroxypropyl betadex, Hydroxypropyl-β-cyclodextrin) is a well-known sugar used in drug delivery, genetic vectors, environmental protection, and the treatment of Niemann-Pick disease type C1 (NPC1). It is an inhibitor of amyloid-β aggregation and widely used drug delivery vehicle to improve stability and bioavailability.
Targets
amyloid-β aggregation [1]
In vitro

HP-β-CD molecules were not only nontoxic to cells, but also greatly inhibited Aβ fibrillization and reduced Aβ-induced toxicity in a concentration-dependent manner. Too low concentrations of HP-β-CD caused insufficient interactions with Aβ, while too high concentrations of HP-β-CD caused HP-β-CD to self-aggregate into inactive species. HP-β-CD interacted preferentially with some of the hydrophobic residues of Aβ, which prevented Aβ oligomers from further growing into mature fibrils via peptide elongation and lateral association[1].

In vivo

HP-β-CD, due to its excellent biocompatibility, has been widely used in drug delivery systems, environmental remediation, food additives, and pharmacotherapy. HP-β-CD can readily cross the BBB and target nerve cells[1]. HP-β-CD is well tolerated in the animal species tested (rats, mice and dogs), particularly when dosed orally, and shows only limited toxicity. After a single 200 mg/kg intravenous dose in rats and dogs, 14C-HP-β-CD was eliminated rapidly (more than 90% in 4 h), almost completely as the intact compound and mostly by renal excretion. The excretion in faeces and expired air was minimal. The plasma elimination half-life was 0.4 h in rats and 0.8 h in dogs. After oral administration of HP-β-CD in both rats and dogs, 86% was excreted via the faeces in both species, where as less than 5% was excreted in the urine. The absolute bioavailability was estimated at 3.3% in the dog and less in the rat. In both rats and dogs following intravenous administration, tissue distribution was limited: in rats the highest concentration was found in the kidney and lung and in dogs, the highest concentrations were in the kidney and the liver. Plasma levels of unchanged HP-β-CD declined rapidly and showed a bi-phasic decline after single intravenous and oral dosing in healthy volunteers. The utility of a 45%w/v HP-β-CD aqueous dosing vehicle in preclinical studies is very common. This vehicle is useful with poorly aqueous drugs[2].

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    SH-SY5Y cell line

  • Concentrations

    25, 50, 125, and 250 μM

  • Incubation Time

    24 and 48 h

  • Method

    Cells that was cultured at 37 ℃ in the mixture of sterile-filtered Eagle's minimum essential medium and Ham's F-12 medium mixed at a 1 : 1 ratio with 10% fetal bovine serum, 1% penicillin/streptomycin and humidified air with 5% CO2 was employed as a model neuron. Cells were cultured to confluence and harvested using 0.25 mg/mL trypsin/EDTA solution. Before adding Aβ1-42 and HP-β-CD, cells were resuspended in Opti-MEM reduced serum medium and counted using a hemocytometer. Cells were then plated in a 96-well tissue culture plate with approximately 100 000 cells per well in 200 μL medium. To determine the cell viability, colorimetric MTT metabolic activity assay was performed. SH-SY5Y cells were cultured in a 96-well plate at 37 1C. Then, Aβ1-42, HP-β-CD and Aβ1-42-HP-β-CD solutions were individually added to the cultured cells, which were then continually cultured for additional 24 h and 48 h. After removing the supernatant of each well, 20 μL of MTT solution (5 mg/mL in PBS) and 100 mL of medium were then added into the systems. After incubation for another 4 h, the formazan crystals were dissolved in dimethyl sulfoxide (150 μL) and the absorbance intensity was measured using a microplate reader at 570 nm.

Selleck's (2-Hydroxypropyl)-β-cyclodextrin (HP-β-CD) has been cited by 2 publications

NT157 Inhibits HCC Migration via Downregulating the STAT3/Jab1 Signaling Pathway [ Technol Cancer Res Treat, 2021, 20:15330338211027916] PubMed: 34238066
Deoxypodophyllotoxin suppresses tumor vasculature in HUVECs by promoting cytoskeleton remodeling through LKB1-AMPK dependent Rho A activatio. [ Oncotarget, 2015, 6(30):29497-512] PubMed: 26470595

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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