2-D08

Catalog No.S8696 Batch:S869602

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Technical Data

Formula

C15H10O5
Molecular Weight 270.24 CAS No. 144707-18-6
Solubility (25°C)* In vitro DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description 2-D08 (2',3',4'-trihydroxy flavone) is a cell permeable, mechanistically unique inhibitor of protein sumoylation.
Targets
sumoylation [2]
In vitro

2-D08, a inhibitor of protein SUMOylation, enhances the all-trans retinoic acid (ATRA)-induced expression of key genes involved in differentiation, proliferation, and apoptosis in non-APL AML cells[1]. 2-D08 inhibits sumoylation by preventing transfer of SUMO from the UBC9-SUMO thioester to the substrate[2]. 

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    U937, HL60, THP1 cell lines

  • Concentrations

    50 µM

  • Incubation Time

    5days, 9 days

  • Method

    Cells are treated for 5 days 2-D08 (50 µM). CD14, CD11b or CD15 expressions are assayed by western bolt or flow cytometry. Morphological changes of cells are shown by May-Grumwald-Giemsa (MGG)-stained. 
Animal Study:

[3]
  • Animal Models

    α2−/− mice (Eight- to 10-week-old mice)

  • Dosages

    30 μM (10 μl)

  • Administration

    Injected on one hemisphere near the hippocampal area

Selleck's 2-D08 has been cited by 19 publications

Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis [ Acta Pharm Sin B, 2024, 14(4):1624-1643] PubMed: 38572092
PQBP3 prevents senescence by suppressing PSME3-mediated proteasomal Lamin B1 degradation [ EMBO J, 2024, 43(18):3968-3999] PubMed: 39103492
YAP O-GlcNAcylation contributes to corneal epithelial cell ferroptosis under cigarette smoke exposure [ Environ Pollut, 2024, 360:124764] PubMed: 39154884
Topotecan and Ginkgolic Acid Inhibit the Expression and Transport Activity of Human Organic Anion Transporter 3 by Suppressing SUMOylation of the Transporter [ Pharmaceutics, 2024, 16(5)638] PubMed: 38794300
Nucleoporin Nup358 drives the differentiation of myeloid-biased multipotent progenitors by modulating HDAC3 nuclear translocation [ Sci Adv, 2024, 10(23):eadn8963] PubMed: 38838144
Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis [ Acta Pharmaceutica Sinica B, 2024, 10.1016/j.apsb.2024.01.006] PubMed: none
SUMOylation of the ubiquitin ligase component KEAP1 at K39 upregulates NRF2 and its target function in lung cancer cell proliferation [ J Biol Chem, 2023, 299(10):105215] PubMed: 37660919
SUMOylation of the ubiquitin ligase component KEAP1 at K39 upregulates NRF2 and its target function in lung cancer cell proliferation [ J Biol Chem, 2023, 299(10):105215] PubMed: 37660919
hnRNP G/RBMX enhances HPV16 E2 mRNA splicing through a novel splicing enhancer and inhibits production of spliced E7 oncogene mRNAs [ Nucleic Acids Res, 2022, 50(7):3867-3891] PubMed: 35357488
Recapitulating early human development with 8C-like cells [ Cell Rep, 2022, 39(12):110994] PubMed: 35732112

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.