(-)-Menthol

Catalog No.S4714 Batch:S471402

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Technical Data

Formula

C10H20O
Molecular Weight 156.27 CAS No. 2216-51-5
Solubility (25°C)* In vitro DMSO 31 mg/mL (198.37 mM)
Ethanol 31 mg/mL (198.37 mM)
Water 7 mg/mL (44.79 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description (-)-Menthol is a levo isomer of menthol and used as a cooling agent that strongly activates TRPM8.
In vitro (-)-Menthol induces cytotoxicity against murine leukemia WEHI-3 cells in vitro in a dosedependent manner. (-)-Menthol inhibits the growth of rat liver epithelial tumor cells and acts as a potent chemopreventive agent during DMBA initiation of rat mammary tumors. (-)-menthol inhibits N-acetyltransferase activity. It also is found to inhibit the DNA topoisomerase I, II alpha and beta and to promote NF-kappaB expression in human gastric cancer SNU-5 cells. Additionally, (-)-menthol induces human promyelocytic leukemia HL-60 cell death through the Ca2+ release from the endoplasmic reticulum[1].
In vivo The effects of (-)-menthol on WEHI-3 cells in vivo (BALB/c mice) were also examined, and it was observed that the Mac-3 and CD11b markers were decreased, indicating inhibition of differentiation of the precursor of macrophage and granulocyte. The weights of liver and spleen samples from mice treated with (-)-menthol were found to be decreased compared to untreated animals. (-)-Menthol has been shown to be toxic in animals, but in humans, it is considered to be safe, with a small cardio-accelerating effect. At high doses (-)-menthol may exert a depressant effect on the central nervous system in rodents. (-)-menthol statistically decreases the weights of the liver and spleen in the examined animals and also decreases the percentage of MAC-3 and CD11b cells in the blood.[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Murine leukemia cell line (WEHI-3)

  • Concentrations

    0, 25, 50, 75 and 100 μM

  • Incubation Time

    24 h

  • Method

    Approximately 2x105 cells (WEHI-3) are incubated in 12-well plates containing medium for 24 h without (control) or with various concentrations (0, 25, 50, 75 and 100 μM) of (-)-menthol. The cells are harvested and washed with PBS before PI staining. The percentage of viable WEHI-3 cells is determined by trypan blue exclusion and flow cytometry.
Animal Study:

[1]
  • Animal Models

    Male BALB/c mice

  • Dosages

    1 mg/kg or 10 mg/kg

  • Administration

    Oral administration

Selleck's (-)-Menthol has been cited by 1 publication

Growth at Cold Temperature Increases the Number of Motor Neurons to Optimize Locomotor Function [ Curr Biol, 2019, 29(11):1787-1799.e5] PubMed: 31130453

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.