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ZZW-115

ZZW-115 is a potent NUPR1 inhibitor with IC50s of 0.42 μM in Hep2G cells and 7.75 μM in SaOS-2 cells. ZZW-115 shows evident antitumor activity through its interaction with NUPR1.

ZZW-115 Chemical Structure

ZZW-115 Chemical Structure

CAS No. 10122-45-9

Purity & Quality Control

Batch: E103701 DMSO]93 mg/mL]false]Water]46 mg/mL]false]Ethanol]2 mg/mL]false Purity: 99.88%
99.88

ZZW-115 Related Products

Biological Activity

Description

ZZW-115 is a potent NUPR1 inhibitor with IC50s of 0.42 μM in Hep2G cells and 7.75 μM in SaOS-2 cells. ZZW-115 shows evident antitumor activity through its interaction with NUPR1.
Targets
NUPR1 [1]
(in Hep2G cells)		 NUPR1 [1]
(in SaOS-2 cells)
0.42 μM 7.75 μM
In vitro
In vitro

ZZW-115 is capable of efficiently killing the pancreatic tumor cell lines by targeting NUPR1 and inhibits the growth of other tumor-derived cells. ZZW-115 induced cell death is the consequence of a decrease of ATP production by the mitochondria accompanied by an increase of mitochondrial ROS production with a transient metabolic shift toward anaerobic glycolysis inhibiting.[1]
Cell Research Cell lines Panc-1 cells
Concentrations 0 μM - 100 μM
Incubation Time 24 h, 72 h
Method

Cells are plated in 96-well plates. Twenty-four hours later, the media are supplemented with 0–100 μM concentration of ZZW-115, and are incubated for another additional 24 or 72 hours. Cell viability is estimated after addition of the CellTiter-Blue viability reagent (Promega) for 3 hours according to the protocol provided by the supplier. Cell viability the concernings normalized with respect to untreated cell rates.
In Vivo
In vivo

ZZW-115 can disrupt tumor growth at low doses and can reduce tumor size for the xenografted tumors, without obvious neurological side effects in the mice. ZZW-115 is a very efficient anticancer drug candidate in vivo on mice because, due mainly to necrosis, it is capable not only of stopping tumor growth but also of decreasing the tumor size until its disappearance.[1]

Chemical Information & Solubility

Molecular Weight 573.97 Formula

C₂₄H₃₄Cl₃F₃N₄S
CAS No. 10122-45-9 SDF --
Smiles Cl.Cl.Cl.CN(C)CCN1CCN(CCCN2C3=CC=CC=C3SC4=C2C=C(C=C4)C(F)(F)F)CC1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 93 mg/mL ( (162.02 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 46 mg/mL

Ethanol : 2 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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