Home PI3K/Akt/mTOR PTEN inhibitor SF1670 For research use only.

SF1670

SF1670 is a highly potent and specific PTEN inhibitor with IC50 of 2 μM.

SF1670 Chemical Structure

SF1670 Chemical Structure

CAS No. 345630-40-2

Purity & Quality Control

Batch: S731001 DMSO]29 mg/mL]false]Ethanol]1 mg/mL]false]Water]Insoluble]false Purity: 99.01%
99.01

SF1670 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human T-cell Proliferation assay In vitro inhibition of human T-cell proliferation, IC50=0.1 μM 11356112
human T-cells Cytotoxicity assay Cytotoxicity measured against human T-cells, CC50=3.5 μM 11356112
Click to View More Cell Line Experimental Data

Biological Activity

Description SF1670 is a highly potent and specific PTEN inhibitor with IC50 of 2 μM.
Targets
PTEN [1]
2 μM
In vitro
In vitro SF1670 shows potent cytotoxicity in HBEC, PC-3, H1299 cells with IC50 of 5 μM, 10 μM, 44 μM, respectively. In a mouse aortic ring matrigel angiogenesis model, SF1670 stimulates the angiogenic processes. [1] SF1670 augments chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils and enhances neutrophil functions. [2]
Kinase Assay PTEN Inhibition Assay
To determine the dose response of potential PTEN inhibitors, doses of test compounds ranging from 1 nM to 250 uM (final reaction mix concentrations) are evaluated in the general PTEN inhibition assay. To obtain performed IC50 data, two separate rounds of the dose response assay are performed. In the first round, PTEN activity is tested in the presence of inhibitor at 10 fold serial dilutions ranging from 1 nM to 250 uM. Once the concentration range is determined, at which PTEN activity changes dramatically, two additional concentration data points within this range are added and the PTEN inhibition assay is then rerun for the second round. The PTEN inhibition IC50 is presented as the inhibitor concentration at which 50% of the PTEN activity. When the assay was run on multiple occasions and gave slightly different IC50 then those are reported as a range of IC50 found.
Cell Research Cell lines Human brain endothelial cells (HBEC), human prostate cancer cells (PC-3) and human non-small cell lung cancer cells (H1299)
Concentrations ~1 mM
Incubation Time 2 hours
Method Cells are plated into 96-well plates in RPMI 1640 medium supplemented with 10% FBS and incubated overnight at 37°C. in an incubator containing an atmosphere of 5% CO2. The following day, the medium is replaced and cells are starved by placing in 100 uL of serum-free medium for 3 hours. Serially diluted test compounds are added to the wells and incubated with the cells for 2 hours at 37°C. Compounds are tested in a range from 1 mM to 0.1 nM depending on solubility. MTT is added to the wells at a final concentration of 5 μg/ml and incubated with the cells for 3 more hours. At the end of the incubation, the medium is aspirated and the MTT stain in the cells is dissolved by the addition of 100 μL DMSO. Optical density of each well is then measured at 570 nm using a SpectroMax Plus spectrophotometric plate reader. The IC50 determination from the data was made using Prism software.
In Vivo
In vivo Pretreatment of SF1670 (500 nM i.v.) augments bacteria-killing capability in neutropenic mice in both peritonitis and bacterial pneumonia, and decreases the mortality of neutropenia-related pneumonia. [2]
Animal Research Animal Models Neutropenic mice
Dosages 500 nM
Administration Tail-vein injection

Chemical Information & Solubility

Molecular Weight 307.34 Formula

C19H17NO3
CAS No. 345630-40-2 SDF Download SF1670 SDF
Smiles CC(C)(C)C(=O)NC1=CC2=C(C=C1)C3=CC=CC=C3C(=O)C2=O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 29 mg/mL ( (94.35 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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