Home TGF-beta/Smad TGF-beta/Smad inhibitor ML347 For research use only.

ML347

Synonyms: LDN-193719

ML347 (LDN-193719) is a selective BMP receptor inhibitor with IC50 of 32 nM for ALK2, >300-fold selectivity over ALK3. Also inhibits ALK1 activity with IC50 of 46 nM.

ML347 Chemical Structure

ML347 Chemical Structure

CAS No. 1062368-49-3

Purity & Quality Control

Batch: S714801 DMSO]10 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.93%
99.93

ML347 Related Products

Signaling Pathway

TGF-beta/Smad Signaling Pathways

TGF-beta/Smad Signaling Pathway

Biological Activity

Description ML347 (LDN-193719) is a selective BMP receptor inhibitor with IC50 of 32 nM for ALK2, >300-fold selectivity over ALK3. Also inhibits ALK1 activity with IC50 of 46 nM.
Targets
ALK2 [1]
(Cell-free assay)		 ALK1 [1]
(Cell-free assay)
32 nM 46 nM
In vitro
In vitro ML347 also shows potency in BMP-responsive C2C12BRA cells with IC50 of 15 nM, and designated an MLPCN probe. ML347 displays high plasma protein binding in human, rat and mouse plasma. [1]

Chemical Information & Solubility

Molecular Weight 352.39 Formula

C22 H16 N4 O 
CAS No. 1062368-49-3 SDF Download ML347 SDF
Smiles COC1=CC=C(C=C1)C2=CN3C(=C(C=N3)C4=C5C=CC=NC5=CC=C4)N=C2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 10 mg/mL ( (28.37 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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