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Ipriflavone (Osteofix)

Ipriflavone (7-Isopropoxyisoflavon) is used to inhibit bone resorption.

Ipriflavone (Osteofix) Chemical Structure

Ipriflavone (Osteofix) Chemical Structure

CAS No. 35212-22-7

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Ipriflavone (Osteofix) Related Products

Biological Activity

Description Ipriflavone (7-Isopropoxyisoflavon) is used to inhibit bone resorption.
Targets
advanced glycation end products (AGE) [2]
In vitro
In vitro Ipriflavone regulates the differentiation and biosynthetic properties of human bone marrow stromal osteoprogenitor cells (BMC) by enhancing the expression of some important matrix proteins and facilitating the mineralization process. [1] Ipriflavone treatment at 10 μM significantly inhibits advanced glycation end products (AGE) enhanced bone resorption in cultured mouse unfractionated bone cells. [2] In addition, Ipriflavone treatment potently enhances calcium uptake by duodenal cells. [3] Ipriflavone inhibits the proliferation and DNA synthesis of MDA-231 cells in a dose-dependent manner, and blocks the ligand-induced phosphorylation of Tyr845 of the EGFR. Ipriflavone treatment at 50 μM does not induce apoptosis of MDA-231 cells. [4]
Cell Research Cell lines MDA-231 cells
Concentrations Dissolved in absolute ethanol, final concentrations ~50 μM
Incubation Time 24, 48, 72 and 96 hours
Method Cells are exposed to various concentrations of Ipriflavone for 24, 48, 72 and 96 hours. Cells are treated with trypan blue to estimate the number of viable cells. For thymidine incorporation analysis, [3H]Thymidine (1 μCi) is added, and cells are harvested onto glass fiber filters 4 hours later. Radioactivity is determined by scintillation counting.
In Vivo
In vivo Oral administration of Ipriflavone at 12 mg but not 6 mg significantly inhibits the development of new osteolytic bone metastases of MDA-231 cells, suppresses the progression of established osteolytic lesions, and reduces the number of osteoclasts adjacent to cancer cells in bone metastases. Consistent with the in vitro activity, Ipriflavone treatment at 6 or 12 mg/day significantly inhibits tumor growth of MDA-231 xenografts with 25% and 45% reductions by volume and 47% and 63% by weight, respectively, compared to untreated mice, and prolongs the life of tumor-bearing mice. [4] LD50: Mice >2.5g/kg (i.p.), >5g/kg (s.c.), >10g/kg (i.g.); Rats >2.5g/kg (i.p.), >5g/kg (s.c.), >10g/kg (i.g.) [5]
Animal Research Animal Models Male athymic nude mice (ICR-nu/nu) injected s.c. or intracardially with MDA-231 human breast cancer cells
Dosages ~12 mg/day
Administration Oral administration

Chemical Information & Solubility

Molecular Weight 280.32 Formula

C18H16O3
CAS No. 35212-22-7 SDF Download Ipriflavone (Osteofix) SDF
Smiles CC(C)OC1=CC2=C(C=C1)C(=O)C(=CO2)C3=CC=CC=C3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 56 mg/mL ( (199.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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