Home PI3K/Akt/mTOR AMPK activator - OXPHOS inhibitor - Apoptosis related activator Lixumistat (IM156)

research use only

Lixumistat (IM156) AMPK activator

Cat.No.S9604

Lixumistat (IM156) is a potent activator of AMPK that increases AMPK phosphorylation. It blocks oxidative phosphorylation (OXPHOS) through the inhibition of complex I and increases apoptosis. This compound ameliorates various types of fibrosis and inhibits tumors.
Lixumistat (IM156) AMPK activator Chemical Structure

Chemical Structure

Molecular Weight: 315.29

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S960401 DMSO]63 mg/mL]false]Ethanol]20 mg/mL]false]Water]Insoluble]false Purity: 99.93%
99.93

Chemical Information, Storage & Stability

Molecular Weight 315.29 Formula

C13H16F3N5O

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 1422365-93-2 -- Storage of Stock Solutions

Synonyms HL156A Smiles FC(F)(F)OC1=CC=C(NC(=N)NC(=N)N2CCCC2)C=C1

Solubility

In vitro
Batch:

DMSO : 63 mg/mL (199.81 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 20 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg
g
μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO
%
% Tween 80
% ddH2O
% DMSO
+
%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC50/Ki
AMPK [1]
OXPHOS [2]
In vitro

Lixumistat (IM156) treatment of RPMCs inhibits HG-induced myofibroblast transdifferentiation and markers of epithelial-mesenchymal transition (EMT). Moreover, this compound ameliorates HG-induced transforming growth factor-β1, Smad3, Snail, and fibronectin expression in the RPMCs via AMPK upregulation.[3] It also decreases complex I-dependent NADH oxidation in a significant, dose-dependent manner.[2]
In vivo

Treatment with Lixumistat (IM156) in vivo exacerbated the memory differentiation of virus-specific CD8+ T cells, resulting in an increase in short-lived effector cells but a decrease in memory precursor effector cells. Thus, this compound impairs the function of virus-specific memory CD8+ T cells, indicating that excessive AMPK activation weakens memory T cell differentiation, thereby suppressing recall immune responses.[1]
References

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Signaling Pathway Map