Home NF-κB NF-κB inhibitor 4'-Hydroxychalcone For research use only.

4'-Hydroxychalcone

Synonyms: P-Cinnamoylphenol

4'-Hydroxychalcone (P-Cinnamoylphenol), found in herbs and spices and tea, is a member of the class of compounds known as retrochalcones. It has diverse biological activities, inhibiting TNFα-induced NF-κB pathway activation in a dose-dependent manner and activating BMP signaling.

4'-Hydroxychalcone Chemical Structure

4'-Hydroxychalcone Chemical Structure

CAS No. 2657-25-2

Purity & Quality Control

Batch: S493702 DMSO]44 mg/mL]false]]]false]]]false Purity: 99.88%
99.88

4'-Hydroxychalcone Related Products

Signaling Pathway

NF-κB Signaling Pathways

NF-κB Signaling Pathway

Biological Activity

Description 4'-Hydroxychalcone (P-Cinnamoylphenol), found in herbs and spices and tea, is a member of the class of compounds known as retrochalcones. It has diverse biological activities, inhibiting TNFα-induced NF-κB pathway activation in a dose-dependent manner and activating BMP signaling.
Targets
NF-κB [2]
In vitro
In vitro The oxidation to 4′-hydroxychalcone was catalyzed by CYP 1A1 and 2C6. It shows estrogenic activity[1]. 4'-hydroxychalcone inhibits TNFα-induced NF-kB pathway activation in a dose-dependent manner. It inhibits TNFα-dependent degradation of IkBα and subsequently prevents p50/p65 nuclear translocation leading to 40-hydroxychalcone-inhibited expression of NF-kB target genes. Inhibition of NF-kB activation by 4'-hydroxychalcone is not leukemia cell-type specific and has no significant effect on non-transformed cell viability. 26S proteasome is a major molecular target of 4'-hydroxychalcone[2].
Cell Research Cell lines K562 cells
Concentrations 20, 24, 28, 32, 40 μM
Incubation Time 2 h
Method

K562 cells are treated with 4'-hydroxychalcone at the different concentrations for 2 h followed by a TNFα-treatment (20 ng/mL) during 6 h.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting
Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer
Gynecologic Oncology Group|National Cancer Institute (NCI)|GOG Foundation
 January 2100 --
NCT04662164 Withdrawn
Type 2 Diabetes Patients
Shanghai HEP Pharmaceutical Co. Ltd.
 December 2025 Phase 1|Phase 2
NCT06177132 Not yet recruiting
Vestibular Disorder
University Hospital Ghent|University Ghent
 November 2025 Not Applicable
NCT03277170 Not yet recruiting
Asthma; Status|Asthma in Children|Asthma Acute|Asthma Attack|Acute Asthma Exacerbation
Vanderbilt University Medical Center
 September 1 2025 Phase 2

Chemical Information & Solubility

Molecular Weight 224.25 Formula

C15H12O2
CAS No. 2657-25-2 SDF --
Smiles C1=CC=C(C=C1)C=CC(=O)C2=CC=C(C=C2)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 44 mg/mL ( (196.2 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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