Home Metabolism phosphatase inhibitor Etidronate For research use only.

Etidronate

Etidronate is a non-aminobisphosphonate, show protein tyrosine phosphatase (PTP) inhibitory activity, used for the treatment of Paget's disease and the prevention of heterotopic ossification.

Etidronate Chemical Structure

Etidronate Chemical Structure

CAS No. 2809-21-4

Purity & Quality Control

Batch: S185701 Water]41 mg/mL]false]DMSO]Insoluble]false]Ethanol]Insoluble]false Purity: 99.17%
99.17

Etidronate Related Products

Biological Activity

Description Etidronate is a non-aminobisphosphonate, show protein tyrosine phosphatase (PTP) inhibitory activity, used for the treatment of Paget's disease and the prevention of heterotopic ossification.
Targets
protein tyrosine phosphatase [6]
In vitro
In vitro Etidronate inhibits directly osteoclastic bone-resorbing activity by pit assay. Etidronate also directly induces apoptosis and disrupts actin rings in osteoclasts. [1]
In Vivo
In vivo Etidronate, when administered s.c. repeatedly at 10 or 40 mg/kg/day, gradually suppresses the adjuvant-induced allodynia In the rats with adjuvant arthritis, as assessed by 10-g von Frey hair. Etidronate (10-40 mg/kg/day) suppresses the adjuvant-induced mechanical allodynia in rat hindpaw. Etidronate (5–10 mg/kg/day) dose-dependently prevents the decrease in bone mineral density (BMD) in the proximal tibia of the arthritic rats. [2] Etidronate inhibits the histidine decarboxylase induction, but not the other inflammatory reactions induced by alendronate. Etidronate (unlike clodronate) also inhibits alendronate-induced BP-line formation in mice (even at 40 mmol/kg). Etidronate (160 mmol/kg) also inhibits the physicochemical changes in the tibia induced by six, weekly injections of alendronate. [3] Etidronate (10 mg/kg) combined with Calcitriol after subtotal nephrectomy (SNx) significantly inhibits thoracic and abdominal aortic calcification 3 weeks after the operation in rats. [4] Etidronate (5 mg/kg or 10 mg/kg) significantly reduces the thoracic and abdominal aortic calcification induced by calcitriol in the renal failure rat. Etidronate (5 mg/kg or 10 mg/kg) also reduces the dysfunction in aortic contraction. Etidronate (5 mg/kg) reverses the reduction in the aortic expression of matrix Gla protein mRNA observed in nephrectomized rats. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02673060 Completed
Bone Metastasis
Osteros Biomedica Ltd
 July 2014 Phase 1
NCT01585402 Completed
Arterial Calcification|CD73 Deficiency
National Heart Lung and Blood Institute (NHLBI)|National Institutes of Health Clinical Center (CC)
 August 20 2012 Phase 2

Chemical Information & Solubility

Molecular Weight 206.03 Formula

C2H8O7P2
CAS No. 2809-21-4 SDF Download Etidronate SDF
Smiles CC(O)(P(=O)(O)O)P(=O)(O)O
Storage (From the date of receipt)

In vitro
Batch:

Water : 41 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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In vivo Formulation Calculator

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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