Home Neuronal Signaling AChR antagonist Tiotropium Bromide hydrate For research use only.

Tiotropium Bromide hydrate

Synonyms: BA 679BR

Tiotropium Bromide hydrate (BA 679BR) is a monohydrate of tiotropium bromide (Spiriva; Tiova; BA 679BR; tiopropium) that is an anticholinergic and bronchodilator and a muscarinic receptor antagonist.

Tiotropium Bromide hydrate Chemical Structure

Tiotropium Bromide hydrate Chemical Structure

CAS No. 139404-48-1

Purity & Quality Control

Tiotropium Bromide hydrate Related Products

Signaling Pathway

AChR Signaling Pathways

AChR Signaling Pathway

Biological Activity

Description Tiotropium Bromide hydrate (BA 679BR) is a monohydrate of tiotropium bromide (Spiriva; Tiova; BA 679BR; tiopropium) that is an anticholinergic and bronchodilator and a muscarinic receptor antagonist.
Targets
AChR [1]
In vitro
In vitro Tiotropium bromide is a potent muscarinic antagonist with equal affinity for M1-, M2- and M3-receptors and is approximately 10-fold more potent than ipratropium bromide. Tiotropium bromide has a potent inhibitory effect against cholinergic nerve-induced contraction of guinea-pig and human airways, that has a slower onset than atropine or ipratropium bromide. Tiotropium bromide, ipratropium bromide and atropine all increase ACh release on neural stimulation and that this effect is washed out equally quickly for the three antagonists. Tiotropium bromide dissociates slowly from M3-receptors (on airway smooth muscle) but rapidly from M2 autoreceptors (on cholinergic nerve terminals). [1] Tiotropium significantly inhibits the release of chemotactic substances by AM, MonoMac6 and A549 cells. [2] Tiotropium bromide inhibits the Th2 cytokine production from PBMCs. [3]
In Vivo
In vivo Tiotropium bromide significantly reduces airway inflammation and the Th2 cytokine production in bronchoalveolar lavage fluid (BALF) in both acute and chronic mouse models of asthma. Tiotropium bromide significantly decreases the goblet cell metaplasia, thickness of airway smooth muscle, and airway fibrosis in a mouse model of asthma. Tiotropium bromide reduces the levels of TGF-beta1 in BALF in a chronic model. [3] Tiotropium inhibits the increase in airway smooth muscle mass, myosin expression, and contractility in a guinea pig model of ongoing allergic asthma. [4] Tiotropium abrogates the LPS-induced increase in neutrophils, goblet cells, collagen deposition and muscularised microvessels in a guinea pig model of chronic obstructive pulmonary disease (COPD), but has no effect on emphysema. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05246046 Completed
Healthy Volunteers
Orion Corporation Orion Pharma
 February 16 2022 Phase 1
NCT04990167 Recruiting
Asthma in Children
Nemours Children''s Clinic
 January 1 2022 Phase 2
NCT05295355 Recruiting
Chronic Obstructive Pulmonary Disease
Guangzhou Institute of Respiratory Disease|Boehringer Ingelheim
 January 25 2021 Not Applicable
NCT04999930 Completed
Chronic Obstructive Pulmonary Disease
National University of Malaysia
 January 10 2020 Not Applicable

Chemical Information & Solubility

Molecular Weight 490.43 Formula

C19H22NO4S2.Br.xH2O
CAS No. 139404-48-1 SDF Download Tiotropium Bromide hydrate SDF
Smiles C[N+]1(C2CC(CC1C3C2O3)OC(=O)C(C4=CC=CS4)(C5=CC=CS5)O)C.O.[Br-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 8 mg/mL ( (16.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Tiotropium Bromide hydrate | Tiotropium Bromide hydrate ic50 | Tiotropium Bromide hydrate price | Tiotropium Bromide hydrate cost | Tiotropium Bromide hydrate solubility dmso | Tiotropium Bromide hydrate purchase | Tiotropium Bromide hydrate manufacturer | Tiotropium Bromide hydrate research buy | Tiotropium Bromide hydrate order | Tiotropium Bromide hydrate mouse | Tiotropium Bromide hydrate chemical structure | Tiotropium Bromide hydrate mw | Tiotropium Bromide hydrate molecular weight | Tiotropium Bromide hydrate datasheet | Tiotropium Bromide hydrate supplier | Tiotropium Bromide hydrate in vitro | Tiotropium Bromide hydrate cell line | Tiotropium Bromide hydrate concentration | Tiotropium Bromide hydrate nmr