Home Cell Cycle APC inhibitor - E3 Ligase inhibitor TAME For research use only.

TAME

Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.

TAME Chemical Structure

TAME Chemical Structure

CAS No. 901-47-3

Purity & Quality Control

Batch: S222501 DMSO]69 mg/mL]false]Water]69 mg/mL]false]Ethanol]3 mg/mL]false Purity: 99.96%
99.96

TAME Related Products

Signaling Pathway

APC Signaling Pathways

APC Signaling Pathway

Biological Activity

Description Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.
Targets
APC [1]
In vitro
In vitro TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. [1] TAME is hydrolysed by trypsin with Km of 0.328 mM. [2] TAME accelerates the ATP hydrolysis process about 12-fold. [3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. [4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. [5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M. [6]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05651620 Recruiting
Calorie Restriction
Tufts University|Pennington Biomedical Research Center|Washington University School of Medicine|Duke University|National Institute on Aging (NIA)
 April 6 2023 --

Chemical Information & Solubility

Molecular Weight 342.41 Formula

C14H22N4O4S
CAS No. 901-47-3 SDF Download TAME SDF
Smiles CC1=CC=C(C=C1)S(=O)(=O)NC(CCCN=C(N)N)C(=O)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 69 mg/mL ( (201.51 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 69 mg/mL

Ethanol : 3 mg/mL


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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