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Rifampin (Rifampicin)

Synonyms: NSC-113926

Rifampin (Rifampicin) is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.

Rifampin (Rifampicin) Chemical Structure

Rifampin (Rifampicin) Chemical Structure

CAS No. 13292-46-1

Purity & Quality Control

Rifampin (Rifampicin) Related Products

Biological Activity

Description Rifampin (Rifampicin) is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.
Targets
RNA polymerase [1]
In vitro
In vitro

Rifampin inhibits IκBα degradation and mitogen-activated protein kinase (MAPK) phosphorylation. Rifampin is found to bind to human MD-2 in a Rifampin and MD-2 concentration-dependent manner. Rifampin inhibits NF-κB activation induced by LPS (20 ng/ml) in a dose-dependent manner, with an IC50 of 44.1 μM in Blue hTLR4 293 cells (A) and immunocompetent microgial BV-2 cell. Rifampin (50 μM) suppresses NF-κB activation at varying LPS doses, and the maximum NF-κB level induced by LPS in the presence of Rifampin (50 μM) is much lower than that in the absence of Rifampin. Rifampin inhibits NO production induced by LPS (200 ng/ml) in a dose-dependent manner in BV-2 cells, with an IC50 of 21.2 μM. Rifampin suppresses LPS induced TNF-α and IL-1β production in both microglia BV-2 and RAW 264.7 macrophage cells. Rifampin-inhibiting innate immune signaling is independent of the pregnane X receptor NR1I2. [1] Rifampin combined with polyester vascular prostheses (PVP) functionalised with cyclodextrin (PVP-CD) results in significant bacterial adhesion reduction and growth inhibition against Gram-positive bacteria. [2] Rifampin (50 μg/mL) significantly reduces the CFU counts of stationary-phase cultures and reduces the CFU counts of the log-phase culture to zero. Rifampin is particularly suitable since it is bactericidal and starts killing M. tuberculosis within an hour of exposure. [3]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05685719 Completed
COVID-19 Pneumonia
Zhejiang ACEA Pharmaceutical Co. Ltd.
 January 4 2023 Phase 1
NCT05635110 Completed
Pain
Vertex Pharmaceuticals Incorporated
 December 15 2022 Phase 1
NCT05098041 Completed
Healthy Volunteers
Takeda
 November 22 2021 Phase 1
NCT04933682 Completed
Healthy
Alexion Pharmaceuticals Inc.
 June 23 2021 Phase 1

Chemical Information & Solubility

Molecular Weight 822.94 Formula

C43H58N4O12
CAS No. 13292-46-1 SDF Download Rifampin (Rifampicin) SDF
Smiles CC1C=CC=C(C(=O)NC2=C(C(=C3C(=C2O)C(=C(C4=C3C(=O)C(O4)(OC=CC(C(C(C(C(C(C1O)C)O)C)OC(=O)C)C)OC)C)C)O)O)C=NN5CCN(CC5)C)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (121.51 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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