Home Transmembrane Transporters Antineoplastic and Immunosuppressive Antibiotics inhibitor - ATPase inhibitor Oligomycin A For research use only.

Oligomycin A

Synonyms: MCH 32

Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.

Oligomycin A Chemical Structure

Oligomycin A Chemical Structure

CAS No. 579-13-5

Purity & Quality Control

Products often used together with Oligomycin A

FCCP

Oligomycin A inhibits ATP synthase and reduces OCR, whereas FCCP uncouples oxygen consumption from ATP production and raises OCR to a maximal value in SK-Mel-28 cells.

Plitzko B, et al. Bio Protoc. 2018 May 20;8(10):e2850.

Oligomycin A Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 cells Function assay 25, 50, 75, 100 μM elevated the glucose uptake level but not dose-dependent 30205572
Vero cells Function assay 0.01, 0.1, 1, and 10 μM a decrease in the viability of Vero cells (~20% reduction) at increasing concentrations. 26859745
ISE6 cells Function assay 0.01, 0.1, 1, and 10 μM a decrease in the viability of ISE6 cells (~60%) at increasing concentrations. 26859745
Click to View More Cell Line Experimental Data

Biological Activity

Description Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1]

In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2]

Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3]

Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]
Cell Research Cell lines H1229
Concentrations 100 ng/ml
Incubation Time 1 h
Method

ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-GCN2 / GCN2 / p-eIF2α / eIF2α / ATF4 / xCT pAkt / Her2 / Raf / Hsp70 / Hsp90 p-T389 S6K1 / S6K1 / p-T172 AMPK / AMPK Cox1p / Cox2p / Cox3p / Cox4p 27708226
In Vivo
In vivo

Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.
Animal Research Animal Models Male Sprague-Dawley rats
Dosages 0.5 mg/kg
Administration i.v.

Chemical Information & Solubility

Molecular Weight 791.06 Formula

C45H74O11
CAS No. 579-13-5 SDF Download Oligomycin A SDF
Smiles CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (126.41 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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