Home DNA Damage/DNA Repair Antineoplastic and Immunosuppressive Antibiotics inhibitor - DNA/RNA Synthesis inhibitor Dacarbazine For research use only.

Dacarbazine

Synonyms: DTIC-Dome, Imidazole Carboxamide,NSC-45388

Dacarbazine is a triazene derivative with antineoplastic activity. Dacarbazine alkylates and cross-links DNA during all phases of the cell cycle, resulting in disruption of DNA function, cell cycle arrest, and apoptosis; used in the treatment of various cancers.

Dacarbazine Chemical Structure

Dacarbazine Chemical Structure

CAS No. 4342-03-4

Purity & Quality Control

Dacarbazine Related Products

Biological Activity

Description Dacarbazine is a triazene derivative with antineoplastic activity. Dacarbazine alkylates and cross-links DNA during all phases of the cell cycle, resulting in disruption of DNA function, cell cycle arrest, and apoptosis; used in the treatment of various cancers.
Targets
Nucleoside antimetabolite/analog [1]
In vitro
In vitro Treatment of Dacarbazine sensitizes melanomas to lysis of peptide-specific CTL and it is is mediated through Fas-independent pathway[2].
Cell Research Cell lines UACC903 (UACC) cells
Concentrations 20 μM
Incubation Time 48 h
Method

Apotosis assay: UACC903 (UACC) cells treated with or without DTIC (20 μM) for 48 h are incubated with agonist anti-Fas Ab, CH-11 (500 ng/ml), for 5 and 16 h, respectively. To distinguish whether death/apoptosis is mediated by FasR, blocking anti-Fas Ab ZB4 (2 mg/ml) is added in the corresponding groups. At the end of incubation, cells are harvested and stained with propidium iodide and FITC-annexin V (BD PharMingen) for detecting apoptosis according to the instruction provided by the company. Ten thousand cells from each group are collected, and dead and apoptotic cells are analyzed without gating using CellQuest software.
Experimental Result Images Methods Biomarkers Images PMID
Western blot ATM / p-ATM / p21(Waf1/Cip1) / Survivin 27632933
Growth inhibition assay Cell viability 21976975
In Vivo
In vivo Treatment of combination of Axitinib and DTIC demonstrates significant antitumor activity against melanoma flank xenografts, reduces tumor cell proliferation and decreases the area of tumor necrosis and increases apoptosis. It also reduces meta-tasis-related factors and prolongs lifespan in mice[3].
Animal Research Animal Models B16F1 melanoma xenograft model(C57BL/6 mice background)
Dosages 80 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03761095 Completed
Leiomyosarcoma
PTC Therapeutics
 March 13 2019 Phase 1

Chemical Information & Solubility

Molecular Weight 182.18 Formula

C6H10N6O
CAS No. 4342-03-4 SDF Download Dacarbazine SDF
Smiles CN(C)N=NC1=C(NC=N1)C(=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 3 mg/mL ( (16.46 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.

Frequently Asked Questions

Question 1:
Could you please help me test some common vehicles for S1221 in vivo experiments?

Answer:
S1221 can dissolve in 0.5% CMC Na at 30mg/ml as a suspension. After stayed for a period of time, the powder will drop down to the bottom of the tube, so please vortex well before use.

Tags: buy Dacarbazine | Dacarbazine ic50 | Dacarbazine price | Dacarbazine cost | Dacarbazine solubility dmso | Dacarbazine purchase | Dacarbazine manufacturer | Dacarbazine research buy | Dacarbazine order | Dacarbazine mouse | Dacarbazine chemical structure | Dacarbazine mw | Dacarbazine molecular weight | Dacarbazine datasheet | Dacarbazine supplier | Dacarbazine in vitro | Dacarbazine cell line | Dacarbazine concentration | Dacarbazine nmr