Ki67 Antibody [J16J15]

Catalog No.: F0126

For research use only.

    Home Primary Antibodies Ki67 Antibody [J16J15]

    Application: Reactivity:

    Experiment Essentials

    WB
    Recommended SDS-PAGE separating gel concentration: 5%.
    Recommended wet transfer conditions: 250 mA, 180 min.

    Usage Information

    Dilution
    1:100 - 1:500
    1:200
    1:250
    1:1000
    Application
    WB, IHC, IF, FCM
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Observed MW
    359 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.
    Positive Control Human tonsil; Human colon carcinoma; Human colon; Human pancreas; Human testis; Human kidney; Human liver; Mouse liver; Mouse spleen; Mouse kidney; Rat spleen; Ramos cell; A549 cell; HAP1 cell; Human cardiac stem cell; Human HEp-2 cell; HCT116 cell
    Negative Control

    Datasheet & SDS

    Biological Description

    Specificity
    Ki67 Antibody [J16J15] detects endogenous levels of total Ki-67 protein.
    Subcellular Location
    Chromosome, Nucleus
    Uniprot ID
    P46013
    Clone
    J16J15
    Synonym(s)
    Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; MKI67
    Background
    Ki-67 is a large nuclear and nucleolar protein encoded by the MKI67 gene and is widely used as a marker of cell proliferation. Structurally, it contains an N-terminal forkhead-associated (FHA) domain, a protein phosphatase 1 (PP1) binding region, central tandem repeats, and a C-terminal leucine/arginine-rich chromatin-binding domain, with multiple isoforms generated through alternative splicing. Ki-67 is expressed in all active phases of the cell cycle (G1, S, G2, and M) but is absent in quiescent (G0) cells, making it a reliable proliferation index in cancer diagnostics. Functionally, it plays critical roles in heterochromatin organization during interphase, assembly of the perichromosomal layer (PCL) during mitosis to prevent chromosome aggregation, and regulation of cell cycle progression through interactions with PP1 and other chromatin-associated proteins.
    References
    • https://pubmed.ncbi.nlm.nih.gov/29322240/
    • https://pubmed.ncbi.nlm.nih.gov/10837136/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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